SAN FRANCISCO (AP) — Investigators are examining the cockpit interaction of two Asiana Airlines pilots who had taken on new roles before the crash of Flight 214 — one of whom had seldom flown a Boeing 777 and an instructor who was on his first training flight.

There were four pilots on board but the National Transportation Safety Board is focusing on the working relationship between Lee Gangkuk, who was landing the big jet for his first time at San Francisco International Airport, and Lee Jeong-Min, who was training him.

While the two men had years of aviation experience, this mission involved unfamiliar duties, and it was the first time they had flown together.

The flight came to a tragic end when the airliner, which came in too low and too slow, crash-landed on Saturday, killing two passengers and injuring many others as it skittered and spun 100 feet.

The pilots were assigned to work together through a tightly regulated system developed after several deadly crashes in the 1980s were blamed in part on inexperience in the cockpit, NTSB chairman Deborah Hersman said Wednesday.

“We are certainly interested to see if there are issues where there are challenges to crew communication, if there’s an authority break in where people won’t challenge one another,” she said.

Pilots are trained to communicate their concerns openly, she said, “to make sure that a junior pilot feels comfortable challenging a senior pilot and to make sure the senior pilot welcomes feedback in a cockpit environment from all members of the crew and considers it.”

Hersman said the pilot trainee told investigators he was blinded by a light at about 500 feet, which would have been 34 seconds before impact and the point at which the airliner began to slow and drop precipitously. She said lasers have not been ruled out. It was unclear, however, whether the flash might have played a role in the crash.

Hersman also said a third pilot in the jump seat of the cockpit told investigators he was warning them their speed was too slow as they approached the runway.

And she said when the plane came to a stop, pilots told passengers to stay seated for 90 seconds while they communicated with the tower as part of a safety procedure. Hersman said this has happened after earlier accidents and was not necessarily a problem. People did not begin fleeing the aircraft until 90 seconds later when a fire was spotted outside the plane.

“Hi, we are at the San Francisco International Airport and we just got in a plane crash and there are a lot of people that need help,” a survivor told an emergency dispatcher in a 911 call released late Wednesday.

“There’s been a crash at San Francisco airport,” another caller reported. “There are people injured on the tarmac, seriously injured. There are no ambulances.”

The 911 dispatcher told the caller that help was on the way.

Hersman stressed that while the trainee pilot was flying the plane, the instructor was ultimately responsible, and thus the way they worked together will be scrutinized.

“That’s what the airline needs to do, be responsible so that in the cockpit you’re matching the best people, especially when you’re introducing someone to a new aircraft,” former NTSB Chairman James Hall said.

Massachusetts Institute of Technology aeronautics professor Mary Cummings said it’s common for two commercial pilots who have never worked together before to be assigned to the same flight.

But she said the military tries to have crews work together more permanently.

“Research would tell you that crew pairing with the same people over longer periods of time is safer,” she said. “When two people fly together all the time, you get into a routine that’s more efficient. You have experience communicating.”

Details emerging from Asiana pilot interviews, cockpit recorders and controltower communications indicate that Lee Gang-kuk, who was halfway through his certification training for the Boeing 777, and his co-pilot and instructor, Lee Jeong-Min, thought the airliner’s speed was being controlled by an autothrottle set for 157 mph.

Inspectors found that the autothrottle had been “armed,” or made ready for activation, Hersman said. But investigators are still determining whether it had been engaged. In the last two minutes, there was a lot of use of autopilot and autothrusters, and investigators are going to look into whether pilots made the appropriate commands and if they knew what they were doing, she said.

When the pilots realized the plane was approaching the waterfront runway too low and too slow, they both reached for the throttle.