Police and volunteers were testing dogs at a Buxton dog kennel Wednesday afternoon after seizing more than 250 dogs Tuesday.

Police worked with the Westbrook Animal Refuge League and the Kennebunk Animal Welfare Society to remove an estimated 50 dogs from the site. The remaining dogs at the kennel were quarantined and under 24-hour watch, while they were being tested for Giardia and Sarcoptic Mange.

“This is the largest seizure ever in Maine,” said Susan Britt, director of operations at the Animal Refuge League in Westbrook.

Buxton police served a search warrant at 7:30 a.m. Tuesday at J’aime Kennel at 35 Paucek Road to search for medical and veterinary records, and to seize animals to test for disease. Police said Monday that several dogs and puppies had been diagnosed with Sarcoptic Mange. Giardia had already been diagnosed in a puppy sold from the kennel.

John Frasca, 53, and Heidi Frasca, 52, both of Paucek Road, were issued 14 summonses for an unlicensed kennel, two for animal cruelty and one for failing to provide necessary medical treatment to animals.

Officer Mike Grovo of Buxton police, the investigating officer, said Wednesday a court appearance had been set for 1 p.m. on Nov. 14 in Biddeford District Court.

Grovo said kennels are required to be licensed by the town and the state. A town license requires an inspection. “We’ve attempted several times to inspect it but have been turned away in the past year,” Grovo said.

A no-tresspassing sign was posted outside a Buxton dog kennel Wednesday after police seized more than 250 dogs Tuesday.This French bull dog was at the Animal Refuge League in Westbrook Wednesday after being seized from a dog kennel in Buxton.Most of more than 250 dogs seized this week remained quarantined at the J’aime Kennel in Buxton Wednesday as animal rescue workers tested them for Giardia and Sarcoptic Mange.